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Modify parameters help
The best target region score value indicates how good the yellow highlighted region is, taking into account the number of target and off-target n-mers.
The closer to 100 the better is the value. In the same way, the custom region score indicates the value of the custom region, represented by the transparent grey rectangle.
Set Custom Region button will activate a draggable and resizable transparent grey rectangle to manually select a custom region.
Change button will recalculate the results using the new parameters chosen. Changing the n-mer size is the slowest step.
Distribution of n-mers help
N-mers mapping to the target/s are shown in blue and to off-targets in red. The yellow area highlights the region with the highest score using the selected parameters
The bottom graph represents in red the score values along the sequence. The score value = 0 is indicated with a green line.
Below this line are represented the regions with more off-targets than targets, and the opposite when the score is above the green line.
Expand graph button will display every n-mer fragment aligned over the query for each subject.
Zoom button will zoom in/out the VIGS map representation.
Best region help
This section shows the best or the custom region sequence in FASTA format.
The custom region will update as the grey selection rectangle is moved.
Sequence overview help
In this section is shown the query sequence, highlighting the best target region in yellow or the custom region in grey.
The custom region will be updated as the grey selection rectangle is moved.
Description of genes mapped help
Number of n-mer matches and gene functional description are shown for each matched gene.
The View link will open a draggable dialog with this information.